Sure, I've done that plenty. I have only used the wet-slide stuff from smallbear, but there are probably some alternatives out there. I usually do about 6-7 thin passes of clear coat. It really looks good that way.
I don't sand between coats, but I do rub the enclosure down gently with a cotton t-shirt (usually the one I'm wearing!) before applying the next coat.
I think any acetate sheet that is very thin would work.